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|Title: ||Rapid screening assay of trimethylaminuria in urine with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry|
|Authors: ||許維儀(Wei-Yi Hsu);羅婉瑜(Wan-Yu Lo);賴建成*;蔡輔仁(Fuu-Jen Tsai)*;蔡長海(Chang-Hai Tsai);蔡育勳;林瑋德(Wei-De Lin);趙美琴(Mei-Chyn Chao)|
|Issue Date: ||2009-08-20 17:30:57 (UTC+8)|
|Abstract: ||This study evaluated the proliferative effects of chishao on RSC96, Schwann cells. A dose-dependent proliferative effect of chishao was obtained by methylthiazol tetrazolium (MTT), proliferating cell nuclear antigen (PCNA) Western blotting, and wound healing assays in Schwann cells administered with chishao (0–500 mg/ml), except at 500 mg/ml concentration. The chishao-treated cells also showed a dose-dependent activated fibroblast growth factor-2 (FGF-2) signaling with increased urokinase plasminogen activator (uPA) and decreased plasminogen activator inhibitor-1 (PAI-1), enhanced proliferative proteins, extracellular signal regulated kinase (ERK) and c-Jun N-terminal kinase (JNK)-signaling. Using mitogen-actvated protein kinase (MAPK)-signaling chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of chishao on RSC cells were identified to be ERK- and JNK- signaling dependent. Based on the results, applying appropriate doses of chishao to Schwann cells would be a potential approach for enhancing neuron regeneration.|
|Relation: ||RAPID COMMUNICATIONS IN MASS SPECTROMETRY12(21):1915~1919|
|Appears in Collections:||[台中附設醫院] 期刊論文|
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