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Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/28345

Title: Crystallization and preliminary X-ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia
Authors: (X.-Y. Lin);(S.-J. Liao);(K.-H. Chin);(H.-L. Shih);沈光漢;(A.H.-J. Wang);周三和(S.-H. Chou)*
Contributors: 健康照護學院二年制呼吸治療學系
Keywords: DFS synthase;RpfF;Stenotrophomonas maltophilia;structural genomics.
Date: 2009
Issue Date: 2010-09-23 19:50:21 (UTC+8)
Abstract: Stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia, with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF (SmRpfF) function via small-molecule interference may constitute a new way of treating S. maltophilia infection. SmRpfF was therefore overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 148.51, c = 122.82 A, and diffracted to a resolution of 2.25 A.
Stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia, with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF (SmRpfF) function via small-molecule interference may constitute a new way of treating S. maltophilia infection. SmRpfF was therefore overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 148.51, c = 122.82 A, and diffracted to a resolution of 2.25 A.
Relation: Acta Crystallographica Section F-Structural Biology and Crystallization Com 65():1021-1023
Appears in Collections:[二年制呼吸治療學系] 期刊論文

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